New approaches in education and health sciences management

New approaches in education and health sciences management

Effect of Arginine on Skin Wound Healing in Male Wistar Rats

Document Type : Original Article

Authors
Sanaz Khodadadi 1
Abdollah Amini 2
Seyed Mohammadreza Seyed Abolghasemi 1
Mohammad Javadi 1
Atarodsadat Mostafavinia 3
1 Department of Anatomy & Cognitive Neurosciences, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahiid Beheshti University of Medical Sciences, Tehran, Iran.
3 Department of Anatomy & Cognitive Neurosciences, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
10.22034/edus.2025.567793.1080
Abstract
Introduction: Arginine is an essential amino acid synthesized from citrulline in healthy individuals and modulates immune function, hormone secretion, and endothelial performance. Arginine and its active metabolites can influence all stages of wound healing, including collagen production, cell proliferation, and immune enhancement.
Materials and Methods: In this study, 24 adult male Wistar rats, with an average weight of 200-250 grams, were anesthetized through intramuscular injection of a ketamine-xylazine mixture. A skin incision, 15 mm in diameter. The rats were divided into four groups: control, 2% topical arginine, 5% topical arginine, and injection arginine, with each group consisting of six rats. After 15 days, the rats were sacrificed and the tissue samples were fixed in 10% formalin. After the tissue processing, stereological studies were performed using a light microscope
Results: In this study, number of fibroblast cells in injection arginine group decreased compared to control, 2% and 5% topical arginine groups. Also, dermis volume in injection arginine group decreased significantly compared to 2% and 5% topical arginine groups and control group, but dermis volume in 2% topical arginine group increased compared to control group.
Conclusion: This study shows a significant difference in the wound healing effect of topical arginine compared to injection arginine.
Keywords
L-Arginine
Skin Wound Healing
Stereology Technique
Rat

Ethics Approval ID: IR.IAU.TMU.REC.1402.112 

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New approaches in education and health sciences management
Volume 3, Issue 1
April 2026
Spring 2026
Pages 24-34

  • Receive Date 08 August 2025
  • Revise Date 13 September 2025
  • Accept Date 14 January 2026