New approaches in education and health sciences management

New approaches in education and health sciences management

Investigating the effect of curcumin on human sperm parameters after freezing and thawing

Document Type : Original Article

Authors
Nila Pourfouladchi 1
Shabnam Movassaghi 2
Shabnam Abdi 1
Mohammad Mahdi Nazarnejad 3
Zahra Nadia Sharifi 4
1 Department of Anatomical Sciences & Cognitive Neuroscience, TeMS.C., Islamic Azad University, Tehran, Iran
2 Department of Anatomical Sciences & Cognitive Neuroscience, TeMS.C., Islamic Azad University, Tehran, Iran; Herbal pharmacology research center, TeMS.C., Islamic Azad University, Tehran Iran
3 Department of Anatomical Sciences & Cognitive Neuroscience, TeMS.C., Islamic Azad University, Tehran, Iran.
4 Department of Anatomical Sciences & Cognitive Neuroscience, TeMS.C., Islamic Azad University, Tehran, Iran; Herbal pharmacology research center, TeMS.C., Islamic Azad University, Tehran Iran
10.22034/edus.2025.568031.1081
Abstract
Background and Aim: In this study, the effect of curcumin, a well-known strong antioxidant, on the parameters of human sperm after freezing and thawing was investigated.
Materials and Methods: In this interventional study, the semen of 10 men referred to Tehran infertility center who had normal sperm parameters according to international health standards were used. The samples were analyzed and monitored according to the standard of the World Health Organization. Each seminal fluid sample was further divided into three parts. The control group was frozen without any intervention. The toxicity test group was mixed with 10 μM curcumin and the experimental group was mixed with 10 μM curcumin and then frozen and thawed, then, the findings were analyzed in SPSS software version 25.
Results: Based on the obtained findings from this study, it was found that in the curcumin freezing group, the viability is significantly more favorable than the control group. (P<0.05) The results also showed that the progressive sperm motility in the freezing group with Curcumin increased significantly and non-progressive motility of sperm significantly decreased. On the other hand, the normal morphology of sperms in the curcumin frozen group did not show a significant difference compared to the control group (P-Value<0.05). the amount of ROS production in the control group after thawing is significantly increased compared to the toxicity and curcumin test group (P<0.001), the amount of lipid peroxidation in the control group after thawing compared to the toxicity and curcumin test group as has increased significantly. (P<0.001)
conclusion: Based on the findings of this study, it can be said that curcumin at a dose of 10 μM can effectively improve the parameters of human sperm after freezing and thawing.
Keywords
Curcumin
Sperm Parameters
Antioxidant Effect

Ethics Approval ID: IR.IAU.PS.REC.1400.366

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New approaches in education and health sciences management
Volume 3, Issue 1
april 2026
Spring 2026
Pages 45-56

  • Receive Date 09 October 2025
  • Revise Date 20 November 2025
  • Accept Date 31 December 2025
  • First Publish Date 31 December 2025
  • Publish Date 21 March 2026