New Approaches in Educational Management and Health Sciences

New Approaches in Educational Management and Health Sciences

Comparison of the Effect of Aerobic and Resistance Training Combined with Black Seed Supplementation on the Expression of AMPKα1 and CAT Genes in the Liver Tissue of Rats with Type 2 Diabetes

Document Type : Original Article

Authors
Mohammad Hossein Lotfi 1
Abbas Mehranpour 2
Mahdi Moradi 3
1 Department of Exercise Physiology, Maha.C., Islamic Azad University, Mahallat, Iran.
2 Department of Physical Education and Sport Sciences, School of Literature and Humanity Sciences, Qom University of Toloe Mehr, Qom, Iran
3 Department of Exercise Physiology, Ar.C., Islamic Azad University, Arak, Iran.
10.22034/edus.2026.598559.1142
Abstract
Introduction and Objective: Type 2 diabetes is associated with impaired metabolic regulation, increased oxidative stress, and inflammation, and can affect the normal function of liver tissue. Therefore, the present study aimed to compare the effect of aerobic and resistance training combined with black seed supplementation on the expression of genes related to inflammatory response and cellular defense pathways in the liver tissue of rats with type 2 diabetes.
Methods: The present study was an experimental animal study. In this study, 56 male Wistar rats were randomly divided into seven groups: healthy control, diabetic control, diabetes + high-intensity interval aerobic training (HIIT), diabetes + resistance training, diabetes + black seed, diabetes + HIIT + black seed, and diabetes + resistance training + black seed. One-way analysis of variance (ANOVA) and Bonferroni's post-hoc test were used for data analysis.
Results: The results showed that type 2 diabetes was associated with a significant decrease in AMPKα1 gene expression and a significant increase in CAT gene expression in liver tissue. The combination of high-intensity interval aerobic training and black seed significantly increased AMPKα1 expression and significantly altered CAT expression.
Conclusion: The results of the present study showed that the combination of high-intensity interval aerobic training and resistance training with black seed supplementation had a more favorable effect on a number of molecular and metabolic indicators compared to single interventions. Therefore, it seems that the combination of exercise training with black seed supplementation could be a more effective approach to improving some metabolic disorders and antioxidant defense associated with type 2 diabetes; however, the difference in effectiveness between the two types of training was not uniform across all indicators, and further studies are needed to confirm the molecular mechanisms of these effects.
Keywords
Aerobic Training
Resistance Training
Black Seed Supplementation
Type 2 Diabetes

Ethics Approval ID: IR.IAU.SARI.REC.1405.240

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New Approaches in Educational Management and Health Sciences
Volume 3, Issue 2
July 2026
Summer 2026
Pages 97-114

  • Receive Date 27 April 2026
  • Revise Date 10 July 2026
  • Accept Date 09 August 2026